(a) Add 5.0 mL Na-citrate (0.05 M, pH 4.8) to a test tube and mix with 1 mL enzyme. (b) Add one filter paper strip and incubate at 50°C for 60 min. (c) Add 3.0 mL DNSA reagent and boil for 15.0 min in boiling water. (d) Add 20 mL distilled water.Mix by completely inverting the tube several times. (e) When pulp has settled well (at least after 10 min), the color formed is measured by ...
Using these shotgun cloning techniques, cellulases were cloned from Cellulomonas fimi Citation [24,25], ... In spite of the great difference in habitat, the cellulolytic enzyme set of A. cellulolyticus is similar to that of the Streptomyces. All three enzyme sets are a mixture of GH5, GH6, GH9, GH12 and GH48 cellulases. ...
Zymography for cellulase was carried out according to the method of Béguin (1983) as follows. The gastropod cellulase is mixed with an equal volume of the loading buffer comprising 20% glycerol, 0.5% SDS, 50 mM Tris–HCl (pH 7.5), and 0.02% BPB (not containing β-mercaptoethanol and urea) and subjected to SDS-PAGE at 4°C. After the electrophoresis, the …
Introduction. The genus Cellulomonas originally described by Bergey et al. (1923) was emended by Clark (1952).The unification of Cellulomonas and Oerskovia was accompanied by the subsequent emendation of the genus Cellulomonas to accommodate organisms with lysine or ornithine as diagnostic diamino acid of the peptidoglycan (Stackebrandt et al., …
This minireview will be limited to "club-shaped" Gram-positive, medically relevant species of the genus Corynebacterium and of the "irregular" Gram-positive coryneforms mostly but not exclusively from the suborder Micrococcineae.Those coryneforms were selected because they most closely resemble Corynebacterium species sensu stricto phenotypically and so can be …
Cellulomonas spp. Description: Cellulomonas is a genus of aerobic, Gram-positive bacteria that are known for their ability to degrade cellulose and other complex polysaccharides. The genus Cellulomonas belongs to the Actinobacteria phylum and includes more than 20 species. Cellulomonas bacteria are found in a variety of environments, including ...
One potential method is microbially catalyzed reduction of Cr(VI) to Cr(III), which was first reported with Pseudomonas spp. (Romanenko and Koren'Ken, 1977). Since then, significant progress has been made towards understanding the processes controlling enzymatic reduction of Cr(VI) in Gram negative bacteria, especially those belonging to the genera …
The majority of cel48 genes are found in Clostridium spp., although Bacteroides cellulosolvens and Ruminococcus albus also have at least one cel48 gene. Also, cel48 genes have been detected in Bacillus, Streptomyces, Cellulomonas, and Eukarya. The cel5 gene is found in a broader range of phylogenetic groups (Pereyra et al. 2010).
2. Morphological and physiological characteristics. Acinetobacter spp. are short, plump, typically 1.0–1.5 μm by 1.5–2.5 μm in size as measured during the rapid phase of their growth but often develop into more coccoid in the stationary phase, usually present in pairs or long chains of variable length (Jung and Park, 2015). Acinetobacter spp. are non-fastidious …
Since C. funkei is described as being urease positive and our isolate was negative in the API Coryne kit, we retested urease production using Christensen's urea slope. The type strain of C. funkei and the clinical isolate gave weak or negative results when tested on three different occasions. The urease test was repeated, with incubation at 24°C, 30°C, and 36°C for 5 days.
The Phyre 2 server [] was used to generate tertiary structure homology models of the four Cellulomonas LPMO catalytic modules, which clearly revealed the conserved beta-sandwich fold (Fig. 2 and Additional file 3: Figure S2).The sequence between strands β1 and β3 of AA10 LPMOs (β1 and β2 in AA9s) contains a highly variable region of loops and helices, …
Cellulomonas fimi, Cellulomonas biazotea, and Cellulomonas flavigena are cellulose-degrading microorganisms chosen to compare the degradation of cellulose. C. fimi degraded 2.5 g/L of cellulose within 4 days, which was the highest quantity among the three microorganisms. ... Microbial fuel cells using Cellulomonas spp. with cellulose as fuel J ...
Cellulomonadaceae, a family within the order Actinomycetales, embraces the genera Cellulomonas, Oerskovia, Paraoerskovia (including Koreibacter), Actinotalea, and Tropheryma.Irrespective of algorithms applied to the set of 16S rRNA gene sequences of type strains, Tropheryma whipplei and Actinotalea fermentans branch deeply and have …
From a purely academic viewpoint, it is ironic that, for an organism that is genetically so well defined, on a par with Escherichia coli, its true habitat and life cycle is so poorly understood. One question that has been raised is whether there is a symbiotic relationship between Bacillus spp. and insects and animals [25]. For insects, in ...
One potential method is microbially catalyzed reduction of Cr(VI) to Cr(III), which was first reported with Pseudomonas spp. (Romanenko and Koren'Ken, 1977).Since then, significant progress has been made towards understanding the processes controlling enzymatic reduction of Cr(VI) in Gram negative bacteria, especially those belonging to the genera …
Both Cellulomonas spp. and Azospirillum spp. exhibited a wide range of pH tolerance. When introduced into sterilized soil, the Cellulomonas sp.-Azospirillum brasilense association was more effective in nitrogen fixation at a pH of 7.0 than at the native soil pH (5.6). This was also true of the indigenous diazotrophic microflora of this soil.
Species identification rates of Corynebacterium spp. isolates were similar or higher for MALDI-TOF MS compared to conventional phenotypic methods (Theel et al. 2012; Vila et al. 2012; ... Aureobacterium species (one isolate), Cellulomonas species (two isolates) Microbacterium species (one isolate) and Pseudoclavibacter species (one isolate).
Volume 4. Atinuke M. Olajide, Gisèle LaPointe, in Encyclopedia of Dairy Sciences (Third Edition), 2022 Microbacterium. They are aerobic, Gram-positive, heat-resistant, non-spore forming rods found in dairy products, belonging to the family Microbacteriaceae.M. lacticum has been isolated from pasteurized milk. Likewise, M. liquefaciens and M. gubbeenense have been isolated from …
2014, Cellulomonas pakistanensis sp. nov., A rod-shaped, motile, facultative anaerobic and moderately halotolerant plant growth promoting actinobacterial strain, designated as NCCP-11T, was isolated from paddy grains. ... 11 isolates were identified as Bacillus spp., 3 asPseudomonas spp., while the remaining three belonged to Cellulomonas ...
Cellulose is the most abundant fibrous component in plant biomass. This polymer component presents a high biotechnological potential mainly for biogas and ethanol production. However, the difficulty to deconstruct cellulose fibers is the main barrier to the use of cellulolytic materials in biotechnological approaches. The production and secretion of enzymes able to …